CAMP Factor Test
This test (named for its discoverers), tests for presence of the CAMP factor protein that is produced by Group B Streptococci. The CAMP factor, in combination with the hemolysis produced by Staphylococcus aureus, produce a dramatically increased zone of hemolysis.
+ = expanded zone of hemolysis where unknown organism grows near Staphylococcus aureus
- = No expansion of hemolysis where unknown organism grows near Staphylococcus aureus
1. Obtain a Blood Agar Plate (BAP).
2. Inoculate the BAP with a line of Staphylococcus aureus.
3. Inoculate with your unknown organism in a line that is perpendicular to the line of S. aureus.
4. Incubate for 24 to 48 hours at the appropriate growth temperature.