CAMP Factor Test

Introduction Results Methods Photos

Introduction

This test (named for its discoverers), tests for presence of the CAMP factor protein that is produced by Group B Streptococci.  The CAMP factor, in combination with the hemolysis produced by Staphylococcus aureus, produce  a dramatically increased zone of hemolysis.

Results

+ = expanded zone of hemolysis where unknown organism grows near Staphylococcus aureus
- = No expansion of hemolysis where unknown organism grows near Staphylococcus aureus

Methods

1. Obtain a Blood Agar Plate (BAP).

2.  Inoculate the BAP with a line of Staphylococcus aureus.

3.  Inoculate with your unknown organism in a line that is perpendicular to the line of S. aureus.

4.  Incubate for 24 to 48 hours at the appropriate growth temperature.

PhotographText Box: Negative CAMP Factor Result

Text Box: Positive CAMP Factor Result